Our study showed the critical role of oxidative damage and inflammation in ethanolinduced hepatotoxicity that markedly was inhibited by. Our study demonstrated that high doses of acarbose, alone or in combination with ethanol, can potentiate ccl. Ethanolinduced ros may come from a number of sources, among which ethanol oxidation by cyp2e1 is a major one. Jan 01, 2002 the results of the present investigation indicate that bile salts modulate hepatic disorders induced by chronic ethanol feeding in rats. Ethanol cytotoxicity to a transfected hepg2 cell line.
Autophagy protects against cyp2e1chronic ethanolinduced. Autophagy reduces acute ethanolinduced hepatotoxicity and. Oct 27, 2003 the purpose of this study was to investigate the antioxidant effects of a hot water extract of panax notoginseng png against chronic ethanol. Acarbose alone or in combination with ethanol potentiates. Can phytochemicals be effective in preventing ethanol. Hepatotoxicity was induced on the rats using ethanol and the levels of serum enzymes such as serum glutamic pyruvic transaminase sgpt, serum glutamic oxaloacetic transaminase sgot, total and conjugated bilirubin were estimated. Forty adult male albino rats were divided into four groups as control, etoh 3 g. Implication of free radical mechanisms in ethanolinduced cellular injury. The objective of the present study is to investigate the protective effect of orally administered rosmarinic acid ra on ethanol etohinduced hepatotoxicity in rat liver. Amelioration of antihepatotoxic effect by lichen rangiferinus against.
The antioxidant effect of the ethanolic root extract of hemidesmus indicus, an indigenous ayurvedic medicinal plant used in soft drinks in india, was studied in rats with ethanolinduced hepatotoxicity. Aug 20, 2016 alcoholic liver disease is a direct result of alcohol induced hepatotoxicity coupled with impaired hepatic regenerative activity. Free fatty acids cannot be metabolized, and the lack of aerobic respiration results. Alcohol enhances hivmediated liver damage and promotes the progression to advanced fibrosis and cirrhosis. Autophagy could mitigate ethanolinduced hepatotoxicity by removing damaged mitochondria and accumulated fatty acids ethanolinduced cell death in vivo is minor, resulting in a modest increase in blood alt in most models of acute or chronic treatment without obvious gross histological changes unless other susceptibility factors exist figs.
Previous studies showed statins reduce lipid peroxidation and attenuate free radical damage and exhibit antioxidant activity against hydroxyl and proxy radicals. Pairfed control rats were fed the diet regimen in parallel with the ethanolfed rats through an indwelling gastric cannula. Dawley rats were given one of the following four diets. In an era of improved survival due to modern antiretroviral therapy, liver disease has become a major cause of morbidity and mortality, resulting in death in 1517% of human immunodeficiency virus hivinfected patients. Substance p blocks ethanolinduced hepatotoxicity sciencedirect. Rats were given carnosine 2 gl in drinking water for 4 weeks and then ethanol was administered orally to rats at a dose of 5 gkg every 12 hours for 3 doses totally binge model. Mice received ethanol 5 gkg bw by gavage every 12 hrs for a total of 3 doses. Hepatotoxicity from hepatic toxicity implies chemicaldriven liver damage. Modulatory effects of viola odorata flower and leaf. Ethanolinduced free radicals and hepatic dna strand breaks are prevented in vivo by antioxidants. Our study demonstrated that high doses of acarbose, alone or in combination with ethanol, can potentiate ccl 4 and ap hepatotoxicity in rats by inducing hepatic cyp2e1. Twentyone patients 43% ingested acetaminophen for therapeutic purposes.
The effects of this lecithin as a hepatoprotective drug on body weight and antioxidant status of ethanolexposed rats were studied. Therefore, we examined the role of pxr in binge etohinduced hepatotoxicity. Antioxidant effect of hemidesmus indicus on ethanolinduced. Antioxidant effect of hemidesmus indicus on ethanol. Recent fasting was more common than recent alcohol use among those who suffered hepatotoxicity after a dose of 4 to 10 g of acetaminophen per day p. Sp was administered to hepg2 human hepatocytes in the presence of ethanol, and also intravenously injected into mice with alcoholic liver damage. Indira please note, due to essential maintenance online purchasing will be unavailable between 08.
We observed statistically significant generation of reactive. The effect of carnosine pretreatment on oxidative stress and. Male wildtype wt andpxrnull mice were orally administered three binge doses of etoh 4. Sp enhances hepatocyte viability in ethanolinduced hepatotoxicity. The present study was designed to evaluate the antioxidant activity of livomap on ethanol induced hepatotoxicity. Implication of free radical during copper mechanisms in ethanol induced cellular injury.
Dec 25, 2007 the antioxidant effect of the ethanolic root extract of hemidesmus indicus, an indigenous ayurvedic medicinal plant used in soft drinks in india, was studied in rats with ethanol induced hepatotoxicity. Effect of lecithin in the treatment of ethanol mediated free radical induced hepatotoxicity. Original article antioxidant and hepatoprotective effect of. Oxidative stress may play a major role in the ethanol. Acute ethanol coingestion confers a lower risk of hepatotoxicity after deliberate acetaminophen overdose. Short communication effect of methanolic extract of. Swiss albino mice were administered with different amounts of cq ranging from human therapeutic equivalent of 360 mgkg body wt. Supplementation with the hydrophobic bile salt cdc aggravates ethanolinduced damage, whereas the administration of the hydrophilic bile salt udc has a protective effect. Group a was used as control while group b was given ethanol at a dose of 0. Protection by hot water extract of panax notoginseng on.
Rosmarinic acid attenuates the hepatotoxicity induced by. Protective effect of quercetin on chloroquineinduced. Modulatory effects of viola odorata flower and leaf extracts. Ethanol treatment for 24 h only reduced hepatocyte viability to a small extent data not shown. In this study, we investigated the effect of carnosine pretreatment on ethanolinduced oxidative stress and hepatotoxicity. Autophagy is an intracellular pathway by which lysosomes degrade and recycle longlived proteins and cellular organelles. Hem 200 mgkg bw was gavage before ethanol administration. Pdf ethanol induced hepatotoxicity in albino rats researchgate. These polyphenols prevent oxygenfree radicalsinduced hepatocyte lethality. The effects of ethanol on autophagy are complex but recent studies have shown that autophagy serves a protective function against ethanolinduced liver injury. Pharmacological evaluation of hepatoprotective activity of. Hepatoprotective effect of ethanol extract from berchemia lineate against ccl4 induced acute hepatotoxicity in mice cong li, litao yi, di geng, yuanyuan han, and lianjin weng pharmaceutical biology downloaded from by university of montreal on 120814. Rats fed ethanol from 21 to days were subjected to one or more episodes of hypoxia 6% o2 in order to determine if ethanol predisposed to centrilobular liver necrosis induced by hypoxia. One important xenobiotic consumed daily by millions of people worldwide is alcohol.
Paracetamol induce hepatotoxicity was evaluated by an increase p ethanol exposed rats were studied. Beneficial effects of rosmarinic acid against alcohol. Jun 15, 2018 to examine the effect of sp on ethanol induced hepatotoxicity, we used in vitro 30,31 and in vivo 32,33 models of ethanol induced liver damage. Used as an additive in gasoline, as a diluent or solvent, in many household products, and in pharmaceuticals its metabolism. Administering ellagic acid to rats with ethanolinduced hepatotoxicity was shown to ameliorate the increased serum levels of aminotransferases, lipid peroxides, and hydroperoxides and also to reduce the elevated hepatic contents of cholesterol, free fatty acids, triglycerides, and phospholipids. Ethanol is the most widely abused drug in the world and its longterm use induces oxidative stress in the liver tissue. We investigated the effects of hericium erinaceus hem on liver injury induced by acute alcohol administration in mice.
Twelve male albino rats of 68 weeks old, weighing 150200 gm each were divided into two groups of 6 rats each. Longterm alcohol consumption induces oxidative stress in the liver due to the imbalance between. Pdf twelve male albino rats of 68 weeks old, weighing 150200 gm each were divided. We observed statistically significant generation of reactive oxygen species. Sp was administered to hepg2 human hepatocytes in the presence of ethanol, and also intravenously injected. However, the mechanisms behind these events are uncertain. Readily diffuses across lipid membranes, accounting for its ubiquitous multiorgan effects. Prevention of ethanolinduced liver injury in rats by an. However, the roles of hem on alcohol induced hepatotoxicity have not been reported. Hepatoprotective and antioxidant activities of grapeseeds against ethanolinduced oxidative stress in rats volume 107 issue 1 abdulahad dogan, ismail celik. May 05, 2019 pdf 1505 k pdf plus 475 k citing articles. Kaempferol kaem could protect the ethanolinduced hepatotoxicity likely by inhibiting the cyp2e1 expression and activity.
The full text of this article is available as a pdf 99k. Can phytochemicals be effective in preventing ethanolinduced. Kaempferols protective effect on ethanolinduced mouse. This study gives the information about hepatoprotective activity of abelmoschus moschatus seed extract against paracetamol and ethanol induced hepatotoxicity.
Antioxidant effect of livomap, a polyherbal formulation on. The effects of ethanol on autophagy are complex but recent studies have shown that autophagy serves a protective function against ethanol induced liver injury. Hepatoprotective effect of ethanol extract from berchemia lineate against ccl4induced acute hepatotoxicity in mice cong li, litao yi, di geng, yuanyuan han, and lianjin weng pharmaceutical biology downloaded from by university of. This study investigated the protective mechanisms of kaempferol on ethanolinduced toxicity by dynamic alteration of sp1, hsp70 and cyp2e1 among the nucleus and different organelles in hepatocytes. In the subgroup of patients that regularly consumed excess ethanol, the risk of. Hepatoprotective effect of alhagi sparsifolia against alcoholic liver. Jan 16, 2008 basic research suggests that acute ethanol consumption at the time of acetaminophen ingestion is capable of protecting against hepatotoxicity. The effect of carnosine pretreatment on oxidative stress. The purpose of this study was to investigate the antioxidant effects of a hot water extract of panax notoginseng png against chronic ethanol. Available formats pdf please select a format to send.
Mechanistic studies have shown that the beneficial effects of these phytochemicals in preventing ethanolinduced hepatotoxicity is mediated by antioxidant, free radical scavenging, antiinflammatory, antifibrotic, antimutagenic, and lipotropic effects. Antioxidant effect of livomap, a polyherbal formulation on ethanol induced hepatotoxicity in albino wistar rats. Alcohol abuse is highly prevalent in hiv infected individuals and hence may be a significant negative cofactor in haart induced hepatotoxicity. Silymarin protects against acute ethanolinduced hepatotoxicity in. There is currently no way to reimburse for the absence of liver function. Pdf twelve male albino rats of 68 weeks old, weighing 150200 gm each were divided into two groups of 6 rats each. Amelioration of alcoholinduced hepatotoxicity by the. The atorvastatin was able to prevent the ethanolinduced hepatotoxicity by decreasing the oxidative stress and inflammation processes. Introduction the ability of aliphatic alcohols to potentiate. Effect of lecithin in the treatment of ethanol mediated. Betaine may protect liver against injury and it may prevent vitamin a depletion.
Rouach, implication of free radical mechanisms in ethanolinduced cellular injury, free. The protective effects of aqueous extracts of the fruit rind of garcinia indica gie on ethanol induced hepatotoxicity and the probable mechanisms involved in this protection were investigated in rats. It was hoped that this model might prove useful in attempts to establish a cyp2e1dependent ethanol hepatotoxicity system, and to evaluate the effect of antioxidants on the ethanol toxicity, in order to establish linkage between cyp2e1, generation of reactive species and ethanol induced hepatotoxicity. From the division of digestive and liver diseases, department of internal medicine, university of texas southwestern medical center, dallas. We investigated whether macroautophagy, an evolutionarily conserved cellular mechanism that is commonly activated in response to stress, could protect liver. Serum enzymes and liver histology was determined in both groups. From the division of digestive and liver diseases, department of internal medicine. Role of ethanol as a cofactor in haart induced hepatic. For example, ethanolinduced hepatotoxicity in animal models requires diets that have a high percentage of the total calories as unsaturated fat. Highly active antiretroviral therapy haart has led to a significant increase in the life expectancy of hiv patients. Antioxidant and antiinflammatory role of zingerone in. Aja 1department of biochemistry ebonyi state university abakaliki, nigeria. The pregnane x receptor pxr, nr1i2 is a xenobioticsensing nuclear receptor that defends against toxic agents. Original article antioxidant and hepatoprotective effect.
We have shown that pxr promotes chronic ethanol etohinduced steatosis. Supplementation with the hydrophobic bile salt cdc aggravates ethanol induced damage, whereas the administration of the hydrophilic bile salt udc has a protective effect. Vitamins c and e are antioxidants that scavenge for free radicals. For example, ethanol induced hepatotoxicity in animal models requires diets that have a high percentage of the total calories as unsaturated fat. It was hoped that this model might prove useful in attempts to establish a cyp2e1dependent ethanol hepatotoxicity system, and to evaluate the effect of antioxidants on the ethanol toxicity, in order to establish linkage between cyp2e1, generation of reactive species and ethanolinduced hepatotoxicity. The objective of the present study is to investigate the protective effect of orally administered rosmarinic acid ra on ethanol etoh induced hepatotoxicity in rat liver. An in vivo model of ethanol ingestion in rats was used to examine tumor necrosis factor production after intravenous injection with lipopolysaccharide or saline solution. Atorvastatin attenuates ethanolinduced hepatotoxicity via. Acarbose alone or in combination with ethanol potentiates the. Liver damage was induced in rats by administering ethanol 5 gkg, 20% wv p. Hepatoprotective and antioxidant activities of grapeseeds. Section of liver disease and nutrition and alcohol research and treatment center, bronx veterans affairs medical center and mount sinai school of medicine, new york, new york 10468. Role of the pregnane x receptor in binge ethanolinduced.
Male wildtype wt and pxr null mice were orally administered three binge doses of etoh 4. Drug induced liver injury is a cause of acute and chronic liver disease the liver plays a central role in transforming and clearing chemicals and is susceptible to the toxicity from these agents. In this study, we investigated the effect of carnosine pretreatment on ethanol induced oxidative stress and hepatotoxicity. The results of the present investigation indicate that bile salts modulate hepatic disorders induced by chronic ethanol feeding in rats. Our aim of the study was to investigate the beneficial effect of zingerone on hepatic oxidative stress and inflammation induced by ethanol in experimental rats.
Besides serving as a substrate for gshrelated enzymes, gsh acts as a free radical scavenger and a regenerator of. Antioxidant effect of livomap, a polyherbal formulation on ethanol induced hepatotoxicity in albino wistar rats kanchana. Modulatory effects of viola odorata flower and leaf extracts upon oxidative stressrelated damage in an experimental model of ethanolinduced hepatotoxicity. Administering 20% ethanol 5 gkg of body weightday for 60 days to male wistar rats resulted in significantly decreased body weight and. Therefore, ra may be a good candidate to protect against ethanol induced hepatotoxicity.
It is fully miscible in water and is lipid soluble. Hepatoprotective effect of ethanol extract from berchemia. A potentiation of ccl4induced hepatotoxicity was observed in rats pretreated with ethanol 18 hr prior to cc14 exposure. Delayed blood clearance and enhanced hepatotoxicity. Beneficial effects of rosmarinic acid against alcoholinduced. Autophagy was found to also be protective against cyp2e1dependent toxicity in vitro in hepg2 cells which express. Ethanol etoh causes a further substantial amount of liver insufficiencies world wide. Its major influence is on the important cause of liver injury. Therefore, ra may be a good candidate to protect against ethanolinduced hepatotoxicity. From the present study, it can be concluded that ethanolinduced stress can be partly prevented by tocopheryl acetate, and showed best result. Amelioration of alcohol induced hepatotoxicity by the administration of ethanolic extract of sida cordifolia linn.
The current study is aimed at rectification of alcohol induced liver damage by the. Lee at the division of digestive and liver diseases, university of texas southwestern medical center, 5323 harry hines blvd. Alcoholic liver disease is a direct result of alcoholinduced hepatotoxicity coupled with impaired hepatic regenerative activity. Short communication effect of methanolic extract of hibiscus. Some adverse effects of ethanol, such as alcohol liver disease, have been linked to diet. Kuntze extract ameliorates chronic ethanolinduced hepatotoxicity in albino rats. Association of acetaminophen hepatotoxicity with fasting. Administering 20% ethanol 5 gkg of body weightday for 60 days to male wistar rats resulted in significantly decreased body weight and increased liverbody weight ratio. The aim of this study was to evaluate protective effect of viola odorata against ethanolinduced hepatotoxicity in wistar rat. The results were compared with the effects of tocopheryl acetate. The pathologic features of alcoholic liver disease develop over prolonged periods, yet the cellular defense mechanisms against the detrimental effects of alcohol are not well understood. Rosmarinic acid ra, a natural polyphenolic compound, shows some biological activities including antioxidant and antiinflammatory activity. Alcohol metabolism potentiates hivinduced hepatotoxicity. The protective effects of aqueous extracts of the fruit rind of garcinia indica gie on ethanolinduced hepatotoxicity and the probable mechanisms involved in this protection were investigated in rats.
The effect of ethanol leaf extract of jatropha curcas on. All patients with hepatotoxicity took more than the recommended limit of 4 gd. From the present study, it can be concluded that ethanol induced stress can be partly prevented by tocopheryl acetate, and showed best result. The effect of ethanol leaf extract of jatropha curcas on chloroform induced hepatotoxicity in albino rats 1 11p. Emran habibi, a b milad arabnozari, a c pedram elahi, a b maryam ghasemi, d fatemeh shaki a c.
The present study was planned to evaluate the hepatoprotective activity of ahplaytab06 in comparison with marketed formulations in three different experimental models, i. To model ethanolinduced damage, hepg2 cells were exposed to 200, 300, or 400 mm ethanol for 48 h, after which, their viability was determined. To examine the effect of sp on ethanolinduced hepatotoxicity, we used in vitro 30,31 and in vivo 32,33 models of ethanolinduced liver damage. A potentiation of ccl4induced hepatotoxicity was observed in rats pretreated with ethanol. Male albino wistar rats were divided into four groups. The role of tumor necrosis factor in acute endotoxin. Our findings showed that ra prevents ethanolinduced oxidantantioxidant imbalance and liver injury in an experimental model of ethanolinduced hepatotoxicity. Evaluation of hepatoprotective activity of abelmoschus.
Our findings showed that ra prevents ethanol induced oxidantantioxidant imbalance and liver injury in an experimental model of ethanol induced hepatotoxicity. Therefore, it may be a useful nutritional agent in the prevention of clinical problems dependent on ethanol. Drug induced hepatotoxicity is the most common reason cited for withdrawal of already approved drugs from the market and accounts for more than 50 percent of cases of acute liver failure in the united states. The present study was aimed to find out the protective effect of quercetin on hepatotoxicity resulting by commonly used antimalarial drug chloroquine cq.
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